Analysis Note

ControlWestern blot: brain and liver. Immunocytochemistry: SH-SY5Y cells.

Application

Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment.
Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA.
Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G.

Optimal working dilutions must be determined by end user.

Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC.

Immunogen

Synthetic peptide from torsinB.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

TorsinB. The antibody reacts with the ~38 kDa protein.

Storage and Stability

Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.